Composite
Part:BBa_K2933185:Design
Designed by: Jian Dong Group: iGEM19_TJUSLS_China (2019-09-16)
T7 promoter+RBS b+linker h+His+Linker a+Sumo+Linker b+AFM-1+T7 terminator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 390
Illegal XbaI site found at 47 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 390
Illegal NheI site found at 167
Illegal NheI site found at 1301 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 390
Illegal BglII site found at 279
Illegal BamHI site found at 478 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 390
Illegal XbaI site found at 47 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 390
Illegal XbaI site found at 47 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
NCBI